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Journal: Stem Cell Research & Therapy
Article Title: Protective effects of mouse bone marrow mesenchymal stem cell-derived extracellular vesicles on radiation-induced epididymal cells damage via USP44/RBM14 axis
doi: 10.1186/s13287-025-04782-9
Figure Lengend Snippet: Characterization of mBMSC and Their EVs. A Flow cytometry histograms showing expression of surface markers. Cells were negative for hematopoietic markers CD14, CD19, CD34, and CD45 (< 0.5%) and positive for mesenchymal markers CD29, CD44, CD73, and CD90 (> 98%). Gray shading represents isotype controls; red lines represent specific antibody staining. B Representative images of lineage-specific staining after differentiation induction: Alizarin Red (osteogenesis), Alcian Blue (chondrogenesis), and Oil Red O (adipogenesis). Scale bars, 100 μm. C Transmission electron microscopy images of EVs showing typical morphology and size. Scale bars represent 0.5 μm (left) and 200 nm (right). D Nanoparticle tracking analysis illustrating extracellular vesicle size distribution and concentration, with a peak around 100 nm. E Western blot detection of typical exosomal markers CD9, CD63, and CD81, confirming vesicle identity
Article Snippet:
Techniques: Flow Cytometry, Expressing, Staining, Transmission Assay, Electron Microscopy, Concentration Assay, Western Blot
Journal: Frontiers in Cellular and Infection Microbiology
Article Title: Gingipains disrupt bone homeostasis via dual regulation of osteogenesis and osteoclastogenesis through exosomal miR-146a-5p/TRAF6 signaling
doi: 10.3389/fcimb.2025.1614126
Figure Lengend Snippet: miR-146a-5p deficiency facilitates the osteoclastic differentiation of RAW264.7 cells in vitro . (A) Heat map diagram of the differential expression of exosomal miRNAs identified by miRNA microarray analysis. (B) Expression of osteoclastic-associated genes ( NFATC1 , CTSK , and TRAP ) in RAW264.7 cells transfected with miR-146a-5p mimics (Mimic group), control mimics (Ctr Mimic group), inhibitor (Inhi group), and control inhibitor (Ctr Inhi group) (n = 6; * P < 0.05). (C) Expression of NFATC1, CTSK, and CD40L/CD154 protein levels in RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi group) (a) . Relative protein expression was quantified by ImageJ software (b) (n = 3; * P < 0.05). (D) TRAP staining of RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi groups). * P < 0.05.
Article Snippet:
Techniques: In Vitro, Quantitative Proteomics, Microarray, Expressing, Transfection, Control, Software, Staining